VariaclePharmaceutical Evidence Platform

Accelerating Drug Approvals with
Causal Inference

Variacle integrates pharmaceutical evidence from multi-center clinical databases to dramatically reduce patient recruitment burden and accelerate the path to drug approval with statistical guarantees.

Example clinical trial: Bisoprolol vs Metoprolol

Total Sample Size Impact

ORIGINAL SAMPLE SIZE

131

65 + 66 patients

POST-VARIACLE SAMPLE SIZE

330

65 + 265 patients

This means ~2.5 times more recruitments for free while increasing to 90% success probability, all from integrating evidence.

Study Design

A randomized, open-label study comparing Bisoprolol with Metoprolol Succinate sustained-release on heart rate and blood pressure in hypertensive patients (CREATIVE Trial - NCT01508325).

  • Exposure: Beta-blocker type (Bisoprolol vs Metoprolol)
  • Duration: 12-week treatment period
  • Sample: Bisoprolol (n₁=65), Metoprolol (n₀=66)

Primary Outcome

Change from Baseline in Mean Ambulatory Diastolic Blood Pressure (DBP) in the last 4 hours after 12-week treatment measured via 24-hour ambulatory blood pressure monitoring (ABPM).

Bisoprolol (n=65):-4.45 mmHg
SD (individual):10.74 mmHg
Metoprolol (n=66):-3.39 mmHg
SD (individual):11.23 mmHg

Sample Size Analysis

Pre-Variacle Distribution

Distribution of mean estimates with SEM = σ/√n for each group.
Bisoprolol: Mean = -4.45 mmHg, SEM = 1.33 (SD = 10.74 for n=65)
Metoprolol: Mean = -3.39 mmHg, SEM = 1.38 (SD = 11.23 for n=66)

-9.0-8.0-7.0-6.0-5.0-4.0-3.0-2.0-1.00.01.02.0Change in Diastolic Blood Pressure (mmHg)DensityBisoprolol (n=65)Metoprolol (n=66)

Bisoprolol (n=65)

Mean: -4.45

SD: 1.33

Sample Size: 65

Metoprolol (n=66)

Mean: -3.39

SD: 1.38

Sample Size: 66

Variacle's - Evidence Integration

Using the eICU Collaborative Research Database

Data Source

eICU Collaborative Research Database - A freely available multi-center database for critical care research containing over 139,000 critical care patient records from 208 hospitals.

Learn more about eICU on Nature →

Distribution Comparison

Pre-Variacle vs Post-Variacle

Pre-Variacle: Traditional Approach

-9.0-8.0-7.0-6.0-5.0-4.0-3.0-2.0-1.00.01.02.0Change in Diastolic Blood Pressure (mmHg)DensityBisoprolol (n=65)Metoprolol (n=66)

Bisoprolol (n=65)

Mean: -4.45

SD: 1.33

Metoprolol (n=66)

Mean: -3.39

SD: 1.38

Post-Variacle: With eICU Evidence Integration

-9.0-8.0-7.0-6.0-5.0-4.0-3.0-2.0-1.00.01.02.0Change in Diastolic Blood Pressure (mmHg)DensityBisoprolol (refined)Metoprolol (refined)

Bisoprolol (refined)

Mean: -4.45

SD: 1.33

Metoprolol (refined)

Mean: -3.08

SD: 0.69

Understanding Statistical Power

Statistical power is the probability that a hypothesis test will correctly detect a true effect when one exists. It represents the probability of rejecting the null hypothesis when it is false (avoiding a Type II error).

Given a desired power level and significance level, we can calculate the required sample size of a control group using the following formula. For a fixed sample size n₁ in the treatment group and control group sample size n₀ with significance level α, power (1-β), common standard deviation σ, and effect size δ:

Sample Size for Control Group (n₀):

n₀ = 1 / (δ² / ((z_α + z_β)² × σ²) - 1/n₁)

Applied Example: CHD Risk Reduction

According to meta-analysis by Law et al. ("Use of blood pressure lowering drugs in the prevention of cardiovascular disease: meta-analysis of 147 randomised trials in the context of expectations from prospective epidemiological studies"), a diastolic blood pressure reduction of δ = 5 mmHg corresponds to approximately a 5% reduction in coronary heart disease (CHD) events.

FALSE DISCOVERY RATE

5%

α = 0.05

DESIRED POWER

90%

Power = 0.90

NEEDED METOPROLOL SAMPLE SIZE

234

n₀ required (90% power)

Variacle's Achievement

By refining the Metoprolol distribution using eICU evidence, Variacle achieves an effective sample size of 265:

neff = σ² / σ²Variacle = 265

Total Sample Size Impact

ORIGINAL SAMPLE SIZE

131

65 + 66 patients

POST-VARIACLE SAMPLE SIZE

330

65 + 265 patients

This means ~2.5 times more recruitments for free while increasing to 90% success probability, all from integrating evidence.